Figure 4. Dual-mode quantitative imaging of the change of oxidative stress in hepatocytes responses to NAC treatment against APAP induced liver injury.

(A,B) Representative fluorescence intensity and FLIM images of cellular GSH or H₂O₂ of the control, APAP, APAP + NAC groups at 30 min after NAC treatment (75 min after APAP administration). All images were recorded at λ_Exc/λ_Em: 850/515 to 620 nm. Scale bar: 20 μm. (C,E) The percentages of GSH or H₂O₂-positive hepatocytes of the control, APAP, APAP + NAC groups. (D,F) Population density modeling of the mean GSH or H₂O₂ intensity per cell in control APAP, APAP + NAC groups. Values are the mean ± s.d. for n = 5 mice; *p < 0.05, compared with APAP groups; ^#p < 0.05, compared with control group.