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. 2017 Mar 28;7:45393. doi: 10.1038/srep45393

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(a) Design of B-5a. A “switched” sxRNA sequence was designed to interact with trigger T-5 (shown in red). The incorporated HSL sequence is shown highlighted in green. This sxRNA includes a sequence intended to act as destabilizer (D2) and a sequence intended to isolate the sxRNA (I2) from preceding 5′ sequence (when in bait-reporter context). Prediction shows both these sequences combining to act as a single destabilizer in the “bait only” context (b) RNAfold prediction for the sequence without T-5, note absence of structured HSL motif. (c) RNAcofold prediction for sequence with T-5, showing reformed HSL structural motif.