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. 2017 Mar 27;17:89. doi: 10.1186/s12887-017-0843-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Ct values distribution of RSV and hMPV positive clinical specimens. Our one-step triplex qRT-PCR results showed that the viral load of the RSV or hMPV-positive samples from clinical specimens varied over a wide range, presenting threshold cycles between 21 and 44. RSV has a maximum occurrence detected between Ct 21 and 25, but hMPV has a maximum occurrence between Ct 31 and 35