Table 4.

Reproducibility of the triplex qRT-PCR assay

	Target	Conc. (copies/reaction)	Number of determinations	Mean Ct	SD	CV (%)
Intra-assay	hMPV	108	3	13.90	0.033	0.24
		102	3	35.69	0.170	0.47
	RSV	108	3	15.80	0.015	0.10
		102	3	39.10	0.112	0.29
Inter-assay	hMPV	108	6	13.89	0.032	0.23
		102	6	36.46	0.863	0.91
	RSV	108	6	16.18	0.430	1.04
		102	6	38.98	0.225	0.58