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. 2017 Mar 28;16:133. doi: 10.1186/s12936-017-1760-9

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression of recombinant PvMSA180. a Expression of recombinant domains of PvMSA180 in a wheat germ cell-free expression system. Arrowheads indicate the four domains of PvMSA180. D1 domain 1, D2 domain 2, D3 domain 3, and D4 domain 4. b Expression and purification of PvMSA180-D1 (GST-fused) and D4 (His-tagged) in E. coli. c Recombinant PvMSA180-D1 and D4 were probed with anti-GST and anti-His antibodies under reducing conditions. Arrowheads indicate the PvMSA180-D1 (~80 kDa) and D4 (~60 kDa) bands. d Antibody recognition of PvMSA180-D1 and D4 compare to PvDBP-RII recombinant proteins using pooled vivax-patient sera (lane P), malaria naïve healthy sera (lane H), mouse immune serum (lane M), and anti-GST or anti-His antibody (lane R)