Fig. 4.
Early biofilm formation by human fecal subclone H30 and subclone H22 strains of ST131 Escherichia coli. BioFilm Control® image analysis software was used and results are expressed as the proportion of immobilized beads relative to reference conditions (% RBI) according to incubation time. Bars represent the standard deviation obtained from three independent experiments performed in duplicate. The biofilm is more fully formed (beads are immobilized) as RBI approaches a value of 1. Significant differences between H22 and H30 using Dunnett’s test are indicated by * (p < 0.05), ** (p < 0.01) and *** (p < 0.001)