Fig. 2.

H₂S is not protective for EC against oxidative stress and chemical hypoxia toxicity. Cytotoxicity assay of HMEC, BTEC and RTEC treated for 24 h with NaHS (1–10–100 µM) and H₂O₂ (200 or 400 µM) (A) or CoCl₂ 100 µM (B) or the combination of the two, evaluated with MTS colorimetric kit (see Section 2). C) Proliferation assay of HMEC, BTEC and RTEC maintained for 72 h with NaHS (1–10–100 µM), evaluated with MTS colorimetric kit. Data from a representative experiment were normalized to the respective CNTRL and are expressed as the mean±S.E.M. Wilcoxon test: *p<0.01, **p<0.0001 vs CNTRL, ^#p<0.001 vs CoCl₂ 100 µM; ^$p<0.0001. CNTRL: DMEM 1% FCS medium.