Figure 6.

AβO binding sites co-localize with NKAα3 in hippocampal neuron cultures. Primary rat hippocampal neurons were obtained from E18 embryos and cultured for 18 days before use in immunofluorescence studies. Mature cultures were incubated with 200 nM AβOs (in total Aβ monomer equivalents, which equals 8 nM of 24mers). Neurons were fixed and double-labeled for AβOs (red) and NKAα3 (green). Overlays show prominent co-localization (gold), consistent with NKAα3 being a major AβO binding protein. Controls with labeled f-actin established that superposition of signals is not caused by bleed-through (not shown). Inset shows co-localization at dendritic spines.