Table 1.
Oligonucleotides used for real-time amplification of L. pneumophila.
| Name | Positions on Gene | Sequence (5’→3’) | Fragment Size (bp) | GenBank Accession Number of Reference Sequence |
|---|---|---|---|---|
| 5SF | 618682–618701 | ACTATAGCGATTTGGAACCA | 104 | CP01760.1 |
| 5SR | 618785–618766 | GCGATGACCTACTTTCGCAT | CP01760.1 | |
| 5SProbe | 618737–618759 | HEX-CCGCGCCAATGATAGTGTGAGGC-BHQ | CP01760.1 | |
| dotAF | 986–1004 | ATTGTCTCGCGCGATTGC | 81 | AF095231 |
| dotAR | 1066–1043 | CCGGATCATTATTAACCATCACC | AF095231 | |
| dotA probe | 1006–1027 | FAM-ATACAGCAAATGTATGTGACTT-MGB | AF095231 |