Fig. 8.

Plants with reduced ABA sensitivity are partially resistant to GEX1A. a, The abi1-1C mutant is partially resistant to PB in seedling establishment compared to wild-type Col-0 plants. Quantification of seedling establishment (seedlings developing a first pair of true leaves) was performed on MS plates supplemented with DMSO (Control, white bars), 0.2 μM GEX1A (black bars) or 1 μM ABA (gray bars) 8 days after the seeds were sown. Values are averages of 3 independent experiments ± SD (n > 100). * indicates a p-value ≤ 0.05 by t-test compared to wild type under the same treatment. b, Photograph of representative seedlings from a. c, The abi1-1C mutant is partially resistant to GEX1A in seed germination. Seeds were stratified for 72 h in cold and seed germination (scored as radicle emergence) was calculated 48 h after transfer of the seeds to the controlled growth condition chamber. Values are average of three independent experiments ± SD (n > 100). * indicates a p-value ≤ 0.05 by t-test compared to wild type under the same treatment. Seeds were sown on MS plates supplemented with DMSO (Control, white bars), 1 μM GEX1A (black bars) and 1 μM ABA (gray bars). d, Photograph of representative seedlings showing sensitivities of mutants to ABA and GEX1A. e, Plants with reduced sensitivity to ABA are partially resistant to PB in root growth assay. Seedlings grown vertically on MS plates for 3 days were transferred to MS plates containing DMSO (Control, white bars), 0.2 μM GEX1A (black bars) or 10 μM ABA (gray bars). Root length was calculated with ImageJ 7 days after the transfer. Values are average of three independent experiments ± SD (n > 10). * indicates a p-value ≤ 0.05 by t-test compared to wild type under the same treatment. f, 5-day-old Arabidopsis Col (0) wild-type and sr45-1 mutant seedlings were transferred onto 1⁄2 MS medium with 0.2 μM GEX1A for 4 days. The position of the root tip of seedlings when they were transferred is shown by the black bars