Fig. 3.

Effects of electroacupuncture (EA) at different frequencies on dorsal root ganglion (DRG) P2X3 receptors of diabetic neuropathic pain (DNP) rats

(a) Representative bright-field micrographs showing P2X3 receptor-immunoreactive (IR) neurons in L4, L5, and L6 DRGs of rats in the normal, DNP, DNP+2 Hz EA, and DNP+100 Hz EA groups. Scale bar=100 μ m. (b) Statistical analyses of L4, L5, and L6 DRG P2X3 receptor-IR neurons. Five non-consecutive sections were analyzed to obtain the average for each rat, and three rats were analyzed for each group. Data are presented as mean±SEM. The data were analyzed using ANOVA. ^** P<0.01, compared with the normal group; ^# P<0.05, ^## P<0.01, compared with the DNP group; ^∆ P<0.05, compared with the DNP+2 Hz EA group