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. 2017 Jan 28;6(4):366–373. doi: 10.1016/j.molmet.2017.01.010

Figure 4.

Figure 4

HFD-TMX astrocyte IKKβ KO mice have decreased hypothalamic astrogliosis and inflammation. (A) Representative images of GFAP-ir in the MBH (top) and ARC (bottom). (B) Relative quantification of GFAP-ir (6 sections per mouse analyzed, n = 3 per group). (C) Total GFAP+ cell counts per unilateral ARC. (D) Quantification of individual cell area based on GFAP-ir. (E) Representative images of Iba1-ir. (F) Relative quantification of Iba1-ir. Hypothalamic expression by qRT-PCR (n = 4–6 per group) of (G) glial markers, (H) cytokines, and (I) neuropeptides. All scale bars are 100 μm. Values are mean ± SEM, Student's t-test, *p < 0.05.