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. 2017 Feb 16;6(4):317–326. doi: 10.1016/j.molmet.2017.02.004

Figure 2.

Figure 2

Scatter plots for identified immune populations. Scatter plots including gating strategy for all identified immune cell sub populations within a representative eWAT sample from the HFS group. A) Leukocytes were identified as CD45+ positive cells of the stromal vascular fraction of cells present in adipose tissue. B) ATMs were gated off the CD45+ leukocytes and identified as being CD64+. Dendritic cells were gated off the CD45+ leukocytes and identified as being CD64−/CD11c+. C) ATMs were further identified as being CD11c− or CD11c+. D) T cells were gated off the CD45+ leukocyte population and identified as CD3+. E) T-cells subpopulation were gated off the CD3+ T-cell population and identified as CD8+, CD4+, or double negative (CD4−/CD8−).