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. 2017 Mar 24;11:6–7. doi: 10.1016/j.ymgmr.2017.03.003

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. S1 — Generation of HSPD1 (D29G)-transgenic mice. (A) NcoI-digested transgene expressing HSPD1 (D29G) is composed of SV40 enhancer, mouse MBP promoter [5], human HSPD1 (D29G) tagged with FLAG at the C-terminus, artificial intron [5], and human chorionic gonadotropin polyadenylation signal. (B) Southern blotting confirmed that HSPD1 (D29G)-transgenic mice contain ~ 15 transgenes per genome. Genomic DNA in a denaturing agarose gel is also shown. (C) Presence of transgenes in transgenic mice was also confirmed by genomic PCR for HSPD1 (D29G). Genomic DNA in a non-denaturing agarose gel is also shown. (D) Immunostaining with an anti-FLAG antibody (green) confirmed that HSPD1 (D29G) is expressed along transgenic mouse corpus callosum. Despite decreased staining intensity (for transgenic mice), MBP expression (red) is also shown.