Figure 4.

Peripheral T-cell responses in rhesus macaques injected with vector. Peripheral T-cell responses to AAV8 capsid and the hLDLR transgene were measured by interferon-gamma (IFN-γ) enzyme-linked immunospot assay (ELISPOT) following i.v. injection with 1.25 × 10¹³ GC/kg of AAV8.TBG.hLDLR. Data presented show the time course of T-cell response and AST levels for macaques (A) 090-0263, (B) 090-0287, (C) 19498, and (D) 20031. The plot includes only T-cell responses that met the positive response criteria, defined when the total spot forming units (SFU) when stimulated with antigen per 10⁶ cells is >55 and three times greater than the medium-only negative control value (no stim).