Fig. 1. Scheme of the TS-FITGE experiment. Briefly, the target protein (colored as bright yellow in the microtube) was thermally stabilized by drug engagement and remained in the soluble fraction after thermal denaturation. The soluble proteome of the vehicle-treated group was conjugated to Cy3 dye and the drug-treated group to Cy5 dye, and the two samples were analyzed by 2D gel electrophoresis. The red and green colored spots indicated the thermal stability shift. The melting curves of each spot were plotted against various temperatures. Candidate protein spots were excised and identified by mass spectrometry.