Fig. 6. Thermostability of MelB_St solubilized in three BTM-C11 stereoisomers (A-BTM-C11, B-BTM-C11 and M-BTM-C11) or DDM. MelB_St was extracted from E. coli membranes using 1.5 wt% individual detergents for 90 min at 0, 45, 55 or 65 °C. Following ultracentrifugation to remove insoluble protein and cellular debris, the soluble MelB_St was separated by SDS-PAGE and visualized by Western blot (a). The amount of soluble MelB_St was expressed as percentage of total MelB_St in the untreated membrane (Memb) and presented as a histogram (b). Error bars, SEM, n = 2.