Fig. 1. (A) TEM image of the AuNSs, the scale bar is 20 nm. (B) Dark field scattering image of the AuNSs, the scale bar is 2 μm. (C) Dark field image of a HSC-3 cell pre-incubated with nuclear-targeted gold nanospheres (NT-AuNSs), the NT-AuNSs are mainly localized at the nuclear region. The white dashed line indicates the cell boundary and the green dashed line indicates the cell nucleus, the scale bar is 10 μm. (D) The optical configuration of the Raman probe, the laser beam from the Raman probe is about 2 mm in size, which roughly covers one well of a 96-well plate. (E) Typical Raman spectra of cells with (1) and without (3) NT-AuNSs, and the spectrum of NT-AuNSs (2).
