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. 2017 Jan;8(1-2):472–483. doi: 10.18632/genesandcancer.132

Figure 3. Luciferase assays of the GT198 5´- and 3´-UTR in transiently transfected HEK293T cells.

Figure 3

A. Relative luciferase activity of the 5′-UTR vectors. 5′UTRc.-115G>A, p.GL3.basic.5′UTR.c.-115G>A; 5′UTRc.-70T>A, p.GL3.basic.5′UTR.c.-70T>A; 5′UTRc.-37A>T, p.GL3.basic.5′UTR.c.-37A>T; 5′UTR.c.-24C>G, p.5′UTRc.-24C>G; 5′UTRWT, p.GL3.basic5′UTRWT. B. Luciferase assays of GT198 p.3′UTRc*24G>A and the respective wildtype construct. 3′UTRc.*24G>A, p.GL3.promoter.3′UTR.c.*24G>A; 3′UTRWT, p.GL3.promoter.3′UTRWT. In (A) and (B), bar graphs show the mean+s.d. (n = 9 (three independent experiments performed in triplicates); pGL3.basic was set to 1; following D’Agostino and Pearson omnibus normality test, one-way ANOVA with post-hoc Tukey’s multiple comparison test was performed, P < 0.05).