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. 2017 Mar 28;15(3):e2000532. doi: 10.1371/journal.pbio.2000532

Fig 6. Reducing dietary nutrient density increases feeding and reduces the expression of tetrahydrobiopterin (BH4)-related enzymes.

Fig 6

(A) Wild-type Canton-S flies maintained for 4 d on standard food (SF, n = 35) versus low-energy food (LEF; 1% sucrose solution, n = 35). LEF treatment significantly increases feeding in the capillary feeder (CAFE) assay. (B) Relative Pu gene expression normalized to three control genes under the same conditions as (A) as measured by quantitative polymerase chain reaction (qPCR). Each bar represents the mean ± standard error of the mean (s.e.m.) for three technical replicates of each of two biological samples (RNA extracted from 25 adult fly abdomens) amplified using two different Pu-specific primer sets. (C) Relative pr gene expression normalized to three control genes under the same conditions as (A) as measured by qPCR. Each bar represents the mean ± s.e.m. for three technical replicates of each of three biological samples (RNA extracted from 25 adult fly abdomens) amplified using two different pr-specific primer sets. Underlying numerical data for this figure can be found here: http://dx.doi.org/10.5061/dryad.8hm82.