Table 2. Binding of 125I-labeled proteins to non-fibrillar substrates in a pulldown assay.
Test Protein | Substrate | Recruitment (mean % ± SD) |
---|---|---|
125I-rVλ6Wil | 25 μg WIL monomer | 0.02 ± 0.014 |
~ 25 μg polystyrene amino particles | 0.01 ± 0.014 | |
25 μg WT mouse liver homogenate | 0.4 ± 0.050 | |
no substrate | 0.02 ± 0.021 | |
125I-AL1κ | 25 μg AL1κ monomer | 4.9 ± 0.311 |
~ 25 μg polystyrene amino particles | 4.04 ± 0.163 | |
25 μg WT mouse liver homogenate | 5.47 ± 0.014 | |
no substrate | 4.03 ± 0.516 | |
125I-MM1κ | 25 μg MM1κ monomer | 0.07 ± 0.042 |
~ 25 μg polystyrene amino particles | 0.01 ± 0.014 | |
25 μg WT mouse liver homogenate | 0.81 ± 0.042 | |
no substrate | 0.32 ± 0.00 |