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. 2015 May 7;8(10):16202–16219. doi: 10.18632/oncotarget.3892

Figure 4. Matrix metalloproteinases (MMP)-mediated extra cellular matrix (ECM) remodelling is important during invasion.

Figure 4

A. The relative mRNA levels of MMP1, 2, 9 and 14, B. protein expression and activities on gelatin zymographs of MMPs in human foreskin keratinocytes (HFK) expressing pBabe (control), early and late passage HFK expressing HPV16 E6/E7 genes. C. Relative mRNA levels of MMP1, 2, 9 and 14; and D. protein levels of MMP1, 2, 14 and extracellular activities of MMP2 and MMP9 after transient knockdown by Scram (control) or MMP14 siRNA molecules in the late passage E6/E7-HFK. E. H&E staining and immunofluorescence detection of BrdU uptake and nuclei (DAPI) on 3D-organotypic rafts established from aforementioned MMP14 knockdown cells. Invasive incidents are indicated by arrows. F. Quantification of number of invasive incidents across the rafts per cm. β-actin served as loading control. Scale bar represent 100 μm. N = 3 independent experiments, mean ± SEM, **p < 0.01 compared to the control, *p < 0.05 compared to the controls, †p < 0.05 compared to the early passage population.