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. 2015 May 7;8(10):16202–16219. doi: 10.18632/oncotarget.3892

Figure 8. p63 regulates the Src/c-Jun/matrix metalloproteinases-14 (MMP14)-mediated extra cellular matrix (ECM) remodelling and invasion.

Figure 8

A. Protein levels of total p63, c-Jun, Src, focal adhesion kinase (FAK), MMP14 and β-actin; and activity of MMP2 as read out of the MMP14 activity after knockdown with Scram (control), total p63 or c-Jun siRNA in late passage human foreskin keratinocytes (HFK) expressing HPV16 E6/E7 gene alongside GFP or constitutively active Src (Src-531) constructs. B. H&E staining showing invasions (arrows) and immunofluorescence detection of BrdU uptake and nuclei (DAPI) on 3D-organotypic rafts established with cells from (A). C. Quantification of number of invasive incidents across the rafts per cm. D. Immunofluorescence detection and quantification (Q-score) of p63 and c-Jun in normal epithelium and tumour areas from HPV positive (+ve) and HPV negative (−ve) oro-pharyngeal squamous cell carcinomas. Scale bar represent 100 μm. N = 3 independent experiments, 5 (HPV+ve) and 5 (HPV−ve) tumour sections, mean ± SEM, ***p < 0.001 compared to the normal areas, **p < 0.01 compared to the controls.