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. 2017 Feb 7;8(10):16275–16292. doi: 10.18632/oncotarget.15181

Figure 2. Live Cell Imaging of H1 Uptake by Endocytosis.

Figure 2

A. Live, unfixed, non-permeabilized CHO cells were incubated with Alexa-488 labeled H1 (5 μg/ml) at 37°C and visually tracked over 6 hours. Before imaging, cells were incubated with DAPI at a high enough concentration (10 μg/ml) to stain nuclei within intact cells. Endosomal vesicles were observed in all 4 repetitions of this study. B. CHO cells were incubated for 3 hours with Alexa-488 H1 (10 μg/ml) at 37°C or 4°C. Vesicle formation can be seen in the 37°C cells while the 4°C cells exhibit a dim peripheral staining. C. Cells incubated with Alexa-488 or unlabeled H1 (10 μg/ml) at 37°C for 17 hours were further stained, during the last 30 minutes prior to microscopy, with 50 nM Lysotracker Red and 100 μg/ml Hoechst 33258, a cell permeable DNA stain.