Figure 2. Dual luciferase reporter gene assays with constructs containing different alleles of MDM4 3′-UTR region or pGL3-control vector (empty) were co-transfected with miR-887-3p mimics or NC-miR in the NSCLC A549 cells.

The data are normalized to the luciferase activity observed with the empty vector, which is set to 1. All experiments were performed in triplicates and each value represents mean ± SD. * P < 0.05 compared with the empty control construct by t-tests. NC-miR, negative control miRNAs; NSCLC, non-small cell lung cancer.