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. 2017 Jan 27;8(10):16430–16444. doi: 10.18632/oncotarget.14857

Figure 2. IL31 inhibits tumor cell invasion and migration.

Figure 2

(A) MC38 murine colon carcinoma cells were stably transfected with a control scrambled or siRNA plasmid against IL31. Left panel: Expression of IL31 was assessed by Western blot analysis using anti-IL31 antibodies. Actin served as the loading control. Right panel: IL31 protein levels were quantified by densitometry analysis. (B, C) The invasion and migration properties of control and siIL31 MC38 cells were evaluated using the Boyden chamber assay. Representative images are shown in (B). Scale bar = 200 μm. Quantification of invading and migrating cells is shown in (C). (D, E) The invasion and migration properties of control and IL31-treated MC38 cells were evaluated using the Boyden chamber assay. Representative images are shown in (D). Scale bar = 200 μm. Quantification of invading and migrating cells is shown in (E). **, 0.01 > p > 0.001; ***p < 0.001.