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. 2017 Jan 27;8(10):16430–16444. doi: 10.18632/oncotarget.14857

Figure 5. IL31 directly inhibits endothelial cell tube formation.

Figure 5

(A) The expression level of IL31RA was evaluated in lysates of HUVECs and MDA-MB-231(MDA) cells (positive control) by Western blot. (B) HUVECs were cultured in Matrigel-coated plates and treated with vehicle control or rhIL31 (100 ng/ml) for 24 hours. The formation of tubes was monitored over time using a time-lapse inverted wide-field microscope (Zeiss Axio observer microscope, Germany). Representative images of the 80 min time-point are shown. Scale bar = 250 μm. The number of bifurcations, nodes, segments, and junctions per field (n = 5 fields/group) were analyzed using ImageJ software, and plotted (C). *p < 0.05; **, 0.01 > p > 0.001.