Figure 1. In vitro and in vivo differentiation potential of partially reprogrammed cells.

(A) Partial iPSCs formed flat colonies, whereas fully reprogrammed iPSCs formed dome-like colonies on feeder cell-layered dishes; scale bar = 100 μm. (B) Partial iPSCs did not form embryoid bodies (EB) using the in vitro differentiation protocol. In contrast, fully reprogrammed iPSCs successfully formed EBs; scale bar = 100 μm. (C) The in vivo differentiation potential of partial iPSCs determined by teratoma formation. Partial iPSCs formed teratomas, but mesodermal tissue was rarely detected. Teratoma tissue sections contained ectodermal and endodermal tissues; scale bar = 100 μm.