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(A–C) Multiplex real-time PCR displayed the expression of all eight specific STS markers from AZFa, AZFb and AZFc regions, containing ZFX/ZFY, SRY, sY254, sY127, sY86, sY134, sY84 and sY255, in the immortalized human Sertoli cells (A). DNA from normal human blood served as a positive control (C) and water substituted for DNA as a negative control (B).
