Table 5. The primer sequences of genes used for real-time PCR.
Genes | Primer sequences | Tm (°C) | |
---|---|---|---|
BMP6 | Forward | AGCAATCTGTGGGTTGTGACT | 60 |
Reverse | GGTAGAGCGATTACGACTCTGTT | ||
BMP4 | Forward | TAGCAAGAGTGCCGTCATTCC | 60 |
Reverse | GCGCTCAGGATACTCAAGACC | ||
SOX9 | Forward | AGCGAACGCACATCAAGAC | 60 |
Reverse | CTGTAGGCGATCTGTTGGGG | ||
GATA1 | Forward | TTGTCAGTAAACGGGCAGGTA | 60 |
Reverse | CTTGCGGTTTCGAGTCTGAAT | ||
FGF2 | Forward | AGTGTGTGCTAACCGTTACCT | 60 |
Reverse | ACTGCCCAGTTCGTTTCAGTG | ||
GATA4 | Forward | GCCTCCTCTGCCTGGTAAT | 60 |
Reverse | CAGTCCCATCAGCGTGTAAA | ||
WT1 | Forward | TGACTCTCCACTCCTCCTCAC | 60 |
Reverse | ACCAACTCTTCCAGGCACAC | ||
LIF | Forward | CCAACGTGACGGACTTCCC | 60 |
Reverse | TACACGACTATGCGGTACAGC | ||
GDNF | Forward | GGCAGTGCTTCCTAGAAGAGA | 60 |
Reverse | AAGACACAACCCCGGTTTTTG | ||
GAPDH | Forward | CAGGAGGCATTGCTGATGAT | 60 |
Reverse | GAAGGCTGGGGCTCATTT |