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. 2017 Jan 27;8(10):16553–16570. doi: 10.18632/oncotarget.14985

Table 5. The primer sequences of genes used for real-time PCR.

Genes Primer sequences Tm (°C)
BMP6 Forward AGCAATCTGTGGGTTGTGACT 60
Reverse GGTAGAGCGATTACGACTCTGTT
BMP4 Forward TAGCAAGAGTGCCGTCATTCC 60
Reverse GCGCTCAGGATACTCAAGACC
SOX9 Forward AGCGAACGCACATCAAGAC 60
Reverse CTGTAGGCGATCTGTTGGGG
GATA1 Forward TTGTCAGTAAACGGGCAGGTA 60
Reverse CTTGCGGTTTCGAGTCTGAAT
FGF2 Forward AGTGTGTGCTAACCGTTACCT 60
Reverse ACTGCCCAGTTCGTTTCAGTG
GATA4 Forward GCCTCCTCTGCCTGGTAAT 60
Reverse CAGTCCCATCAGCGTGTAAA
WT1 Forward TGACTCTCCACTCCTCCTCAC 60
Reverse ACCAACTCTTCCAGGCACAC
LIF Forward CCAACGTGACGGACTTCCC 60
Reverse TACACGACTATGCGGTACAGC
GDNF Forward GGCAGTGCTTCCTAGAAGAGA 60
Reverse AAGACACAACCCCGGTTTTTG
GAPDH Forward CAGGAGGCATTGCTGATGAT 60
Reverse GAAGGCTGGGGCTCATTT