Skip to main content
. 2017 Feb 3;8(10):16605–16620. doi: 10.18632/oncotarget.15073

Figure 3. GD2 expression in GBM and non-GBM cell lines.

Figure 3

In order to carry out a preliminary screen of a panel of both GBM and non-GBM cell lines, qualitative PCR (qPCR) was carried out to asses mRNA expression of the key enzyme responsible for the synthesis of disialogangliosidase 2 (GD2). The expression levels of β1,4-N-acetylgalactosaminyltransferase (β4GANT1) mRNA transcript was assessed in n = 2 non-GBM lines (HT29 and A549), n = 2 commercially available GBM lines (A152 and U251), n = 4 primary GBM-patient derived lines (MZ-327, MZ-18, RN1spheroid and JK2), n = 2 recurrent GBM lines (MZ-256 and MZ-304) and a glioma initiating cell line (GIC1080Sp) relative to the cervical cancer cell line, HeLa. As shown (A) RN1spheroid, MZ-256 and MZ-304 have greatest β4GANT1 expression. These highlighted lines were assessed by flow cytometry using a GD2-FITC tagged primary antibody (B) showing positive GD2 antigen presentation on the surface of these cell types (representative flow of n = 4).