Figure 2. Establishment of miR-375 expressing cell lines with pCDH-CMV-MCS-EF1-GFP-T2A-Puro vector.

(A) pCDH-CMV-MCS-EF1-GFP-T2A-Puro plasmid map; (B) Infected cells with GFP were detected by inverted microscope. Scale bars = 100 μm. (C) Infection efficiency showed by flow cytometry. (D) Expression level of miR-375 quantified by qRT-PCR (normalized by U6. mean ± SE are shown; n = 3. **p < 0.01; *p < 0.05; ns, no significance).