Table 6. Primer sequences and amplicons length.

Gene	Primer sequence	Fragment size (bp)
HER2 long	Fw-CCAGGGTGTTCCTCAGTTGT Rev-TCAGTATGGCCTCACCCTTC	295
HER2 short	Fw- CCAGGGTGTTCCTCAGTTGT Rev-GGAGTTCCTGCAGAGGACAG	126
MYC long	Fw TGGAGTAGGGACCGCATATC Rev-ACCCAACACCACGTCCTAAC	264
MYC short	Fw-GGCATTTAAATTTCGGCTCA Rev-AAAAGCCAAATGCCAACTT	128
BCAS1 long	Fw-GGGTCAGAGCTTCCTGTGAG Rev CGTTGTCCTGAAACAGAGCA	266
BCAS1 short	Fw-GGGTCAGAGCTTCCTGTGAG Rev-TATCATGCCTTGGAGAACCA	129
PI3KCA long	Fw-CTC CACGAC CAT CATCAGGT Rev- CGAAGGTCACAAAGTCGTCT	274
PI3KCA short	Fw-CTCCACGACCATCATCATCAGGT Rev-TGGTTATTAATGAGCCTCACGG	129