Figure 6. Relative levels of indicated cytokines in CM of co-culture of breast tumor cells under PRP supplementation and HUVEC.

(A–E) The conditioned medium was recovered to measure protein secretion by flow cytometer in co-cultured cells in pg/ml). (F–J) VEGF concentration in CM after 8–16-hour culture measured by flow cytometry (human VEGF flex set’, BDTM cytometric bead array, CBA, BD Biosciences, USA). Values are the means ± SEM of duplicate determinations in co-cultures from all specimens collected from patients with fibroadenoma, phyllodes, luminal A and B, and HER2+ *p ≤ 0.05, **p ≤ 0.001.