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. 2017 Jan 25;8(10):16912–16924. doi: 10.18632/oncotarget.14818

Figure 5. MHY2013 promotes adipose tissue browning and increases adiponectin levels.

Figure 5

The mice were orally treated with the vehicle (water) or 5 mg/kg/day of MHY2013 for three weeks (n = 5). A. In adipose tissue B. 3T3-L1 adipocytes and C. primary adipocyte from subcutaneous fat, mRNA expression of browning markers (UCP1, CIDEA, PGC-1α, CD137, and SLC27A1) was examined by qRT–PCR. D. In adipose tissue, mRNA expression of adiponectin was evaluated by qRT–PCR. E. Serum adiponectin levels were analyzed using the Mouse Adiponectin ELISA Kit. F. In 3T3-L1 cells, mRNA expression of adiponectin was evaluated by qRT–PCR. In adipose tissue, the mRNA levels of G. fatty acid oxidation-related genes (ACOX1, CPT1, HMGCS2, and PDK4) and H. inflammatory cytokines (MCP1 and TNF-α) were analyzed by qRT–PCR. For mouse studies, *, p < 0.05 vs. db/m; **, p < 0.01 vs. db/m; ***, p < 0.001 vs. db/m; #, p < 0.05 vs. db/db; ##, p < 0.01 vs. db/db; ###, p < 0.001 vs. db/db. For cell experiments, *, p < 0.05 vs. CON; **, p < 0.01 vs. CON; ***, p < 0.001 vs. CON.