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. 2017 Feb 9;8(10):16988–17001. doi: 10.18632/oncotarget.15217

Figure 6. NAC inhibited remifentanil-induced NR2B phosphorylation, NR1 phosphorylation and PKCγ phosphorylation in ipsilateral spinal cord dorsal horn.

Figure 6

(A) Western blotting for p-NR2B, p-NR1, p-PKCγ and β-actin resulted in products of 180, 105, 78 and 43 kDa. (B–D) Densitometric quantification of p-NR2B, p-NR1, p-PKCγ immunoreactivity on Western blots. β-actin was used as a loading control (n = 5). The ipsilateral lumbar spinal cord was collected and analyzed 2 h after intraperitoneal injection. Values expressed as mean ± SD. The treatment style was the same as described in Figure 3. Significant difference was revealed after One-way ANOVA (*P < 0.05 compared with group C+Veh, +P < 0.05 compared with group I+Veh, &P < 0.05 compared with group R+Veh, Bonferroni post hoc tests).