Figure 3. Immunosuppressive property of surgery-induced M-MDSCs and Treg.

A. M-MDSCs were sorted from lung cancer patients (n=16) during perioperative period and arginase activity was detected. B. CD3+ cells derived from lung cancer patients (n=16) were stimulated with anti-CD3 mAb in the presence of M-MDSCs. C. The Treg were sorted from lung cancer patients (n=16) and used for total RNA isolation and the expression of Foxp3 was assessed by quantitative real-time PCR. The concentration of PD-1+ D. and CTLA-4+ cells E. in Treg was analyzed from lung cancer patients (n=16) during perioperative period.