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. 2017 Feb 4;8(10):17216–17228. doi: 10.18632/oncotarget.15303

Figure 2. Detection of pro-apoptotic, anti-apoptotic and apoptotic protein in C4-2B and DU145 PCa cells by immunoblotting.

Figure 2

A. Expression of pro- and anti- apoptotic proteins in C4-2B cells after treatment with RES (47μM), DTX (10nM) and combination (RES+DTX (20μM, 10nM) after 48 h, represents up-regulation of pro-apoptotic proteins (BAX, BID and BAK), cleaved PARP, and down- regulation of anti-apoptotic markers (BCL-2, BCL-XL, and MCL-1). Similar expression of pro- and anti-apoptotic proteins were found in DU145 cells after treatment with RES (35μM); DTX (31nM) and combination (RES+DTX (22μM, 10nM) for 48h treatment. The representative immunoblots are shown with their respective cell lines. B. Densitometry of the pro- and anti- apoptotic proteins blots. GAPDH antibody was used as internal control for each sample. Data are represented as a mean ± standard error of the mean of three independent experiments and determined by the unpaired t- test (*P < 0.01; **P < 0.001).