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. 2017 Feb 4;8(10):17216–17228. doi: 10.18632/oncotarget.15303

Figure 3. Validation of gene expression for pro-apoptotic, PARP and anti-apoptotic markers in PCa cells A.

Figure 3

C4-2B and B. DU145 by qRT-PCR. PCa cells were treated with RES, DTX and combination (RES+DTX) for 48 h. Quantitative RT-PCR analysis showed fold change expression relative to control cells for pro-apoptotic markers (BAX, BID, and BAK), PARP and anti-apoptotic markers (BCL-2, BCL-XL, and MCL-1). Data were normalized to 18S expression, and the experiments were repeated three times. Data are presented as the Mean±SEM, and asterisks indicate significance determined by student t-test (*P < 0.01; **P < 0.001).