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. 2016 Dec 4;8(10):17347–17359. doi: 10.18632/oncotarget.14468

Figure 3.

Figure 3

Comparisons of VEGF, NF-κB and p-IκB/IκB protein expressions in each group by Western blotting (A, Comparisons of VEGF, NF-κB and p-IκB/IκB Protein expression in the control group and DM + CI group; B, Comparisons of VEGF, NF-κB and p-IκB/IκB Protein expression in DM + CI groups. Compared with the Vector group, protein expression of VEGF, NF-κB and p-IκB/IκB were increased in the pcDNA-ANRIL group, while protein expression of VEGF, NF-κB and p-IκB/IκB were decreased in the shANRIL group and the PDTC group. Compared with the pcDNA-ANRIL group, protein expression of VEGF, NF-κB and p-IκB/IκB were decreased in the pcDNA-ANRIL + PDTC group; Relative expression of protein were showed in column chart, data were showed in mean ± standard deviation). Notes: VEGF, vascular endothelial growth factor; NF-κB, nuclear factor-κB; The Vector group, injected with 800 ng/kg empty plasmid; The shANRIL group, injected with 800 ng/kg lncRNA ANRIL knockout plasmid; The PDTC group, injected with 800 ng/kg NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC); The pcDNA-ANRIL + PDTC group, injected with PDTC after lncRNA ANRIL overexpression; DM, diabetes mellitus; CI, cerebral infarction; *, compared with the control/ Vector group, P < 0.05.