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. 2016 Dec 4;8(10):17347–17359. doi: 10.18632/oncotarget.14468

Figure 7.

Figure 7

Comparisons of endothelium dependent MVD in each group by double staining (× 400) (A, endothelium dependent MVD in the Vector group; B, endothelium dependent MVD in the pcDNA-ANRIL group; C, endothelium dependent MVD in the shANRIL group; D, endothelium dependent MVD in the PDTC group; E, endothelium dependent MVD in the pcDNA-ANRIL + PDTC group. Compared with the Vector group, endothelium-dependent vessels were increased in the pcDNA-ANRIL group, while endothelium-dependent vessels were decreased in the shANRIL group and the PDTC group. Compared with the pcDNA-ANRIL group, endothelium-dependent vessels were increased in the pcDNA-ANRIL group.). Notes: MVD, microvessel density; CD31, differentiation 31; PAS, para-amiuosalicylic acid; PDTC, pyrrolidinedithiocarbamic acid; The Vector group, injected with 800 ng/kg empty plasmid; The shANRIL group, injected with 800 ng/kg lncRNA ANRIL knockout plasmid; The PDTC group, injected with 800 ng/kg NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC); The pcDNA-ANRIL + PDTC group, injected with PDTC after lncRNA ANRIL overexpression; DM, diabetes mellitus; CI, cerebral infarction.