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. 2017 Mar 28;12(3):e0174355. doi: 10.1371/journal.pone.0174355

Fig 3. Testosterone hydroxylation is perturbed in CAR-null mice in a gender-specific manner.

Fig 3

(A) Testosterone hydroxylation was determined in male and female WT and CAR-null mice as described in the Materials and Methods. Data are presented as mean specific activity (μmol/min/mg protein) ± SEM (n = 5). (B) Ratio of 6α/15α-hydroxytestosterone as a biomarker of CYP sexual dimorphism in the liver. An aindicates a significant difference between WT male and Cyp2b9/10/13-null male mice, bindicates a significant difference between WT female and Cyp2b9/10/13-null female mice, cindicates a significant difference between male and female WT mice and dindicates a significant difference between the male and female Cyp2b9/10/13-null mice. Statistical differences were determined by two-way ANOVA followed by Fisher’s LSD as the post-hoc test in (A) and one-way ANOVA followed by Fisher’s LSD in (B). A letter without an asterisk indicates a significance of p < 0.05, asterisk indicate significance of *p<0.01, **p<0.001, and *** p<0.0001, respectively.