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. 2017 Mar 28;12(3):e0174413. doi: 10.1371/journal.pone.0174413

Fig 6. ΔMax* binds E-box and non-specific DNA sequences as a heterodimer with Max*.

Fig 6

(A) Far-UV CD spectra of ΔMax* (open triangles), Max* (open diamonds), ΔMax*/Max* (black diamonds) and the arithmetic sum of the individual spectra (gray diamonds). (B) Thermal denaturations of ΔMax* (open triangles), Max* (open diamonds) and ΔMax*/Max* (black diamonds) recorded by monitoring the CD signal at 222 nm and the arithmetic sum of the individual denaturations (gray diamonds). The melting temperatures (T°) are indicated by the dotted lines. (C) Titration of fluorescently labeled E-box (black circles) and non-specific (open circles) sequences with ΔMax*/Max*. (D) Populations of Max*/Max* (blue) and ΔMax*/Max* (red) with the E-box (full line) and the non-specific (dotted line) sequences as a function of the total dimeric concentrations.