Table 1. Results of the analysis of the titration of the E-box and non-specific DNA with the b-HLH-LZs of Max, ΔMax and c-Myc.
Max* | ΔMax* | ΔMax*/Max* | ΔMax*/c-Myc* | c-Myc* | ||||||
---|---|---|---|---|---|---|---|---|---|---|
E-box | NS | E-box | NS | E-box | NS | E-box | NS | E-box | NS | |
1/KBinding (10−9) | 4.8 | 23 | 80 | N/D | 4 | 40 | 10 | 11 | 70 | 280 |
1/KDimer (10−9) | 8.6 ± 1.2 | 55 ± 7 | 220 ± 20 | N/D | 120 ± 10 | 460 ± 34 | 17 ± 2 | 38 ± 5 | 0.48 ± 0.5 | 8.5 ± 3 |
Δr max | 0.053 ± 0.001 | 0.053 ± 0.001 | 0.116 ± 0.003 | N/D | 0.063 ± 0.001 | 0.058 ± 0.001 | 0.045 ± 0.001 | 0.048 ± 0.001 | 0.058 ± 0.001 | 0.110 ± 0.002 |
NS, Non-specific DNA; Δr max, Anisotropy ([Dimer]→∞).