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. 2017 Mar 28;12(3):e0174694. doi: 10.1371/journal.pone.0174694

Fig 3. Overall expression levels of the mouse Sigmar1 splice variant mRNAs.

Fig 3

Expression levels (-1/ΔC(t)) of the mouse Sigmar1 splice variant mRNAs is plotted across tissues and brain regions using MS excel. ΔC(t) values were determined as: ΔC(t)variant − Normalized factor (NF) [33] from at least three independent samples in one experiment. PFC: prefrontal cortex. SYBR green qPCR was performed as described in Materials and Methods. All the primer sequences and qPCR conditions are listed in S1 Table.