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. 2017 Mar 28;12(3):e0174694. doi: 10.1371/journal.pone.0174694

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© 2017 Pan et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — Flag-tagged mMOR-1 and HA-tagged mSigmar1 variants were transiently co-transfected into HEK293 cells. Co-IP was performed as described in Materials and Methods. Briefly, cleared whole cell lysate was incubated with EZview Red Anti-Flag or anti-HA Affinity gels overnight. After washing, the tagged proteins on the affinity gels were eluted with 3xFlag or HA peptide, and the elutes were analyzed in Western blot. Top panel: Immunoblot (IB) with anti-HA antibody using elutes from immunoprecipitation (IP) with EZview Red Anti-Flag Affinity Gel. Bottom panel: IB with anti-Flag antibody using elutes from IP with EZview Red Anti-HA Affinity Gel. The representative blots from two independent experiments were shown.