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. 2016 Dec 20;97(6):412–421. doi: 10.1111/iep.12212

Figure 1.

Figure 1

Upregulation of the expression and methylation levels of the ABCB1 gene in clinical lung adenocarcinoma samples. (a) RT‐PCR analysis of ABCB1 expression in human lung adenocarcinoma samples (tumour) and normal lung tissues (normal). ***P < 0.001. (b) Western blot analysis of ABCB1 expression in different tissues, including human lung adenocarcinoma tissues (T) and normal lung tissues (N). GAPDH is included as loading control. (c) Quantitative analysis results of Western blot. (d) Immunochemical detection results of ABCB1 in normal and tumour tissues. (e) The methylation levels of ABCB1 downstream promoter in human lung adenocarcinoma tissues and normal tissues were detected using bisulphite sequencing. (f) Methylation sites of ABCB1 gene. **P < 0.01.