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. 2016 Dec 20;97(6):412–421. doi: 10.1111/iep.12212

Figure 3.

Figure 3

Pretreatment of DNA methyltransferase (DNMT) inhibitor 5‐azacytidine (5‐Aza‐C)‐induced hypomethylation of the ABCB1 gene and decreased expression of ABCB1 protein in A549 cells. (a) Bisulphite sequencing was performed to detect the methylation levels of ABCB1 downstream promoter in A549 cell after 5‐Aza‐C treatment. (b) Methylation sites of ABCB1 gene. (c) Western bolt analysed the expression of ABCB1 in A549 cells after 5‐Aza‐C treatment. (d) Quantitative analysis results of Western blot. **P < 0.01.