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. 2016 Dec 20;97(6):412–421. doi: 10.1111/iep.12212

Figure 5.

Figure 5

Overexpression of the ABCB1 gene increased DDP resistance of A549 cells. (a) Western blot analysed ABCB1 expression in A549 cells after pcDNA4.1‐ABCB1 transfection. (b) Quantitative analysis results of Western blot. (c) After transfection of pcDNA4.1‐ABCB1 for 24 and 48 h, inhibition of A549 cell viability was analysed using CCK‐8 assay. (d) The cell proliferation of A549 transfected with pcDNA4.1‐ABCB1 was analysed using CCK‐8 assay. (e) EdU assay was used to detect A549 cell proliferation after 5‐Aza‐C treatment. (f) Representative apoptosis assay using flow cytometry in different groups. (g) Hoechst staining of apoptosis. *P < 0.05. **P < 0.01.