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Vesicular membranes 1–10 μm in diameter self-assemble from amphiphilic molecules like phospholipids, as shown in the freeze-fracture image (left panel). When dried, the vesicles fuse and flatten to form multilamellar structures (right panel). Each of the layers is the dimension of a single lipid bilayer ~5 nm thick. In the freeze-fracture method, lipid bilayers are split within the plane of the hydrocarbon chains, not along the head groups.
